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Although a second application of CNO failed to induce a second increase in Ca 2+ in half of the responsive ND neurons, in ND neurons that exhibited a second response to CNO, PPADS reduced the peak area of the CNO-induced Ca 2+ rise by 53%, supporting the idea of ATP-mediated signalling from GLUTag cells to neurons (Fig

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We would like to thank Marianne Seierstad Andresen and Marie- Christine Mowinckel for their assistance with the TaqMan, western blot and multiplex ELISA